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ABOUT US
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SERVICE
SERVICE
Custom peptide
1. Isotope Labeling
Labeling types: 13C/15N full labeling, deuteration (2H), site-specific isotopes (Lys/Arg, etc.)
Inventory coverage: 15 kinds of stable isotope-labeled amino acids in stock (e.g., 13C6-Arg, 15N2-Lys, D10-Leu)
Technical highlights: Stable isotope labeling technology, enabling multiple isotope labeling
2. Fluorescent Labeling
Dye library: CY3/CY5/CY5.5/CY7, FITC, FAM, TAMRA series
Labeling strategy: N-terminal/C-terminal site-specific labeling
Technical highlights: >90% labeling efficiency
|
Fluorescent dye |
Characteristics |
Color |
Excitation wavelength (nm) |
Emission wavelength (nm) |
|
CY3 |
High fluorescence intensity, good photostability, good cell permeability, suitable for various biological experiment labeling |
Orange-red |
550 |
570 |
|
CY5 |
Near-infrared fluorescence, strong tissue penetration, strong fluorescence signal, low background interference, often used in in vivo imaging |
Red |
649 |
670 |
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CY5.5 |
Longer excitation and emission wavelengths, more suitable for deep tissue imaging, good light and chemical stability |
Deep red |
675 |
700 |
|
CY7 |
Near-infrared fluorescence, strong tissue penetration, high fluorescence quantum yield, high detection sensitivity |
Near-infrared |
750 |
773 |
|
FITC |
High fluorescence quantum yield, chemically active, can covalently bind to a variety of biomolecules |
Green |
495 |
520 - 530 |
|
FAM |
Good water solubility and stability, often used for nucleic acid labeling, excitation and emission wavelengths are similar to FITC |
Green |
494 |
520 |
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TAMRA |
Good photostability, high fluorescence intensity, often used as a FRET acceptor dye |
Orange |
555 |
580 |
3. Biotinylation
Technical highlights: Significantly improves detection sensitivity, HPLC purity >98%, can also be combined with fluorescent labeling (such as CY5), isotope labeling (such as 13C), polyethylene glycol (PEG), etc.
4. Protein Conjugation
Carrier protein: KLH (strong immunogenicity), BSA (excellent stability), OVA (commonly used in animal experiments)
Conjugation methods: Glutaraldehyde crosslinking, SMCC chemical conjugation, NHS ester directional linking
5. PEGylation
PEG molecular weight: 2kDa-40kDa (linear/branched optional)
Modification site: N-terminal, C-terminal or Lys side chain site-specific modification
6. Post-translational Modification Mimicry (PTM Mimicry)
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Modification type |
Technical description |
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Lactylation |
Lys site-specific lactic acid group introduction |
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Methylation |
Lys/Arg site mono/di/trimethylation |
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Phosphorylation |
Ser/Thr/Tyr phosphorylation |
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Sulfation |
Tyr sulfation (simulating natural heparin binding site) |
We provide high-quality peptides with two, three, four, and five phosphorylation sites. With mature synthesis and purification technology and a constantly striving elite team, Gu Bio has become a trustworthy peptide supply Brand.
Successful cases:
Sequence NH2-TERD(pS)D(PT)DVEEDSRPPGRPAEVHLERAQPFGFID(pS)D(pS)DAEEEYCONH2, four phosphorylation modification sites.
HPLC analysis:
MS analysis:
Address: Model B204, B205-1, Hi-tech Capital City High-end Equipment Incubation Base, No. 23, Hangbu Road, Baiyan Science Park, Hi-Tech Zone, Hefei City
Sales Tel: 19856166839; 15705607678; 17354056623
Fax: 0551-62869631
E-mail: sales@bankpeptide.com
